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whole transcriptome sequencing  (Illumina Inc)


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    Structured Review

    Illumina Inc whole transcriptome sequencing
    Whole Transcriptome Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 21390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptome+sequencing+library+preparation/TruSeq+RNA+Library+Preparation+Kit+v2/10__61373_slash_gp025a__0047-340-21-30
    Average 99 stars, based on 21390 article reviews
    whole transcriptome sequencing - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: The impact of Sarcocystis infection on lamb flavor metabolites and its underlying molecular mechanisms
    Article Snippet: .. The transcriptome sequencing library was prepared using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, United States), following standard protocols for mRNA enrichment, fragmentation, cDNA synthesis via reverse transcription, adapter ligation, and PCR amplification. .. The library quality was evaluated with an Agilent 2100 Bioanalyzer, and paired-end sequencing (PE150) was conducted on the Illumina NovaSeq 6000 platform ( ).

    Article Title: Draft genome assembly and annotation of Haematococcus lacustris strain Liv1, an industrial astaxanthin-producing microalga.
    Article Snippet: .. For transcriptome sequencing, stranded cDNA libraries were generated using the Illumina Truseq Stranded RNA LT kit. mRNA was purified from 1 μg of total RNA using magnetic beads containing poly-T oligos. mRNA was fragmented and reverse transcribed using random hexamers and SSII (Invitrogen) followed by second-strand synthesis. ..

    Article Title: Draft genome assembly and annotation of Haematococcus lacustris strain Liv1, an industrial astaxanthin-producing microalga
    Article Snippet: Fragments were treated with end repair, A-tailing, and ligation of Illumina-compatible adapters (IDT) using the KAPA-HyperPrep kit (KAPA Biosystems). .. For transcriptome sequencing, stranded cDNA libraries were generated using the Illumina Truseq Stranded RNA LT kit. ..

    Article Title: Single-cell genomics reveals complex microbial and viral associations in ciliates and testate amoebae
    Article Snippet: A Qubit 3.0 fluorometer (Invitrogen) was used to measure the DNA concentration. .. Sequencing libraries for the transcriptome samples were prepared using the Nextera XT DNA Library Preparation Kit (Illumina). ..

    Article Title: Single-cell genomics reveals complex microbial and viral associations in ciliates and testate amoebae.
    Article Snippet: A Qubit 3.0 fluorometer (Invitrogen) was used to measure the DNA concentration. .. Sequencing libraries for the transcriptome samples were prepared using the Nextera XT DNA Library Preparation Kit (Illumina). ..

    Article Title: 5′LysTTT tRNA fragments support survival of botulinum-intoxicated neurons by blocking ferroptosis
    Article Snippet: Botulinum neurotoxins (BoNTs) block cholinergic signaling at neuromuscular junctions, inducing transient muscle paralysis while avoiding neuronal death.. However, the mechanism(s) underlying these dual features are yet unknown.. Here, we report accumulation of 5’Lys transfer RNA fragments (tRFs) in both BoNT/A-intoxicated cultured human neuroblastoma cells and submandibular glands from BoNT/A-intoxicated rodents.

    Article Title: miR-29a-3p serves as a potential biomarker for diagnosing spinal tuberculosis: a comprehensive analysis integrating clinical data and molecular markers.
    Article Snippet: .. 2.2 MicroRNA Transcriptome Sequencing RNA library construction was performed using the Illumina TruSeq Small RNA Sample Prep Kit. .. RNA integrity was assessed using the Agilent 2100 Bioanalyzer system (RIN ≥ 7.0) following quantification with Qubit 2.0.

    Article Title: Co-exposure to polystyrene microplastics and glyphosate induces gut microbiota dysbiosis and cognitive impairment in honeybees.
    Article Snippet: • PS and GLY co-exposure showed more severe impacts on honeybee survival

    Sample Prep:

    Article Title: The impact of Sarcocystis infection on lamb flavor metabolites and its underlying molecular mechanisms
    Article Snippet: .. The transcriptome sequencing library was prepared using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, United States), following standard protocols for mRNA enrichment, fragmentation, cDNA synthesis via reverse transcription, adapter ligation, and PCR amplification. .. The library quality was evaluated with an Agilent 2100 Bioanalyzer, and paired-end sequencing (PE150) was conducted on the Illumina NovaSeq 6000 platform ( ).

    Article Title: miR-29a-3p serves as a potential biomarker for diagnosing spinal tuberculosis: a comprehensive analysis integrating clinical data and molecular markers.
    Article Snippet: .. 2.2 MicroRNA Transcriptome Sequencing RNA library construction was performed using the Illumina TruSeq Small RNA Sample Prep Kit. .. RNA integrity was assessed using the Agilent 2100 Bioanalyzer system (RIN ≥ 7.0) following quantification with Qubit 2.0.

    Article Title: Co-exposure to polystyrene microplastics and glyphosate induces gut microbiota dysbiosis and cognitive impairment in honeybees.
    Article Snippet: • PS and GLY co-exposure showed more severe impacts on honeybee survival

    cDNA Synthesis:

    Article Title: The impact of Sarcocystis infection on lamb flavor metabolites and its underlying molecular mechanisms
    Article Snippet: .. The transcriptome sequencing library was prepared using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, United States), following standard protocols for mRNA enrichment, fragmentation, cDNA synthesis via reverse transcription, adapter ligation, and PCR amplification. .. The library quality was evaluated with an Agilent 2100 Bioanalyzer, and paired-end sequencing (PE150) was conducted on the Illumina NovaSeq 6000 platform ( ).

    Reverse Transcription:

    Article Title: The impact of Sarcocystis infection on lamb flavor metabolites and its underlying molecular mechanisms
    Article Snippet: .. The transcriptome sequencing library was prepared using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, United States), following standard protocols for mRNA enrichment, fragmentation, cDNA synthesis via reverse transcription, adapter ligation, and PCR amplification. .. The library quality was evaluated with an Agilent 2100 Bioanalyzer, and paired-end sequencing (PE150) was conducted on the Illumina NovaSeq 6000 platform ( ).

    Article Title: Draft genome assembly and annotation of Haematococcus lacustris strain Liv1, an industrial astaxanthin-producing microalga.
    Article Snippet: .. For transcriptome sequencing, stranded cDNA libraries were generated using the Illumina Truseq Stranded RNA LT kit. mRNA was purified from 1 μg of total RNA using magnetic beads containing poly-T oligos. mRNA was fragmented and reverse transcribed using random hexamers and SSII (Invitrogen) followed by second-strand synthesis. ..

    Adapter Ligation:

    Article Title: The impact of Sarcocystis infection on lamb flavor metabolites and its underlying molecular mechanisms
    Article Snippet: .. The transcriptome sequencing library was prepared using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, United States), following standard protocols for mRNA enrichment, fragmentation, cDNA synthesis via reverse transcription, adapter ligation, and PCR amplification. .. The library quality was evaluated with an Agilent 2100 Bioanalyzer, and paired-end sequencing (PE150) was conducted on the Illumina NovaSeq 6000 platform ( ).

    Polymerase Chain Reaction:

    Article Title: The impact of Sarcocystis infection on lamb flavor metabolites and its underlying molecular mechanisms
    Article Snippet: .. The transcriptome sequencing library was prepared using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, United States), following standard protocols for mRNA enrichment, fragmentation, cDNA synthesis via reverse transcription, adapter ligation, and PCR amplification. .. The library quality was evaluated with an Agilent 2100 Bioanalyzer, and paired-end sequencing (PE150) was conducted on the Illumina NovaSeq 6000 platform ( ).

    Amplification:

    Article Title: The impact of Sarcocystis infection on lamb flavor metabolites and its underlying molecular mechanisms
    Article Snippet: .. The transcriptome sequencing library was prepared using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, United States), following standard protocols for mRNA enrichment, fragmentation, cDNA synthesis via reverse transcription, adapter ligation, and PCR amplification. .. The library quality was evaluated with an Agilent 2100 Bioanalyzer, and paired-end sequencing (PE150) was conducted on the Illumina NovaSeq 6000 platform ( ).

    Generated:

    Article Title: Draft genome assembly and annotation of Haematococcus lacustris strain Liv1, an industrial astaxanthin-producing microalga.
    Article Snippet: .. For transcriptome sequencing, stranded cDNA libraries were generated using the Illumina Truseq Stranded RNA LT kit. mRNA was purified from 1 μg of total RNA using magnetic beads containing poly-T oligos. mRNA was fragmented and reverse transcribed using random hexamers and SSII (Invitrogen) followed by second-strand synthesis. ..

    Article Title: Draft genome assembly and annotation of Haematococcus lacustris strain Liv1, an industrial astaxanthin-producing microalga
    Article Snippet: Fragments were treated with end repair, A-tailing, and ligation of Illumina-compatible adapters (IDT) using the KAPA-HyperPrep kit (KAPA Biosystems). .. For transcriptome sequencing, stranded cDNA libraries were generated using the Illumina Truseq Stranded RNA LT kit. ..

    Article Title: 5′LysTTT tRNA fragments support survival of botulinum-intoxicated neurons by blocking ferroptosis
    Article Snippet: Botulinum neurotoxins (BoNTs) block cholinergic signaling at neuromuscular junctions, inducing transient muscle paralysis while avoiding neuronal death.. However, the mechanism(s) underlying these dual features are yet unknown.. Here, we report accumulation of 5’Lys transfer RNA fragments (tRFs) in both BoNT/A-intoxicated cultured human neuroblastoma cells and submandibular glands from BoNT/A-intoxicated rodents.

    Purification:

    Article Title: Draft genome assembly and annotation of Haematococcus lacustris strain Liv1, an industrial astaxanthin-producing microalga.
    Article Snippet: .. For transcriptome sequencing, stranded cDNA libraries were generated using the Illumina Truseq Stranded RNA LT kit. mRNA was purified from 1 μg of total RNA using magnetic beads containing poly-T oligos. mRNA was fragmented and reverse transcribed using random hexamers and SSII (Invitrogen) followed by second-strand synthesis. ..

    Magnetic Beads:

    Article Title: Draft genome assembly and annotation of Haematococcus lacustris strain Liv1, an industrial astaxanthin-producing microalga.
    Article Snippet: .. For transcriptome sequencing, stranded cDNA libraries were generated using the Illumina Truseq Stranded RNA LT kit. mRNA was purified from 1 μg of total RNA using magnetic beads containing poly-T oligos. mRNA was fragmented and reverse transcribed using random hexamers and SSII (Invitrogen) followed by second-strand synthesis. ..

    DNA Library Preparation:

    Article Title: Single-cell genomics reveals complex microbial and viral associations in ciliates and testate amoebae
    Article Snippet: A Qubit 3.0 fluorometer (Invitrogen) was used to measure the DNA concentration. .. Sequencing libraries for the transcriptome samples were prepared using the Nextera XT DNA Library Preparation Kit (Illumina). ..

    Article Title: Single-cell genomics reveals complex microbial and viral associations in ciliates and testate amoebae.
    Article Snippet: A Qubit 3.0 fluorometer (Invitrogen) was used to measure the DNA concentration. .. Sequencing libraries for the transcriptome samples were prepared using the Nextera XT DNA Library Preparation Kit (Illumina). ..

    RNA Library Preparation:

    Article Title: 5′LysTTT tRNA fragments support survival of botulinum-intoxicated neurons by blocking ferroptosis
    Article Snippet: Botulinum neurotoxins (BoNTs) block cholinergic signaling at neuromuscular junctions, inducing transient muscle paralysis while avoiding neuronal death.. However, the mechanism(s) underlying these dual features are yet unknown.. Here, we report accumulation of 5’Lys transfer RNA fragments (tRFs) in both BoNT/A-intoxicated cultured human neuroblastoma cells and submandibular glands from BoNT/A-intoxicated rodents.

    RNA Sequencing:

    Article Title: 5′LysTTT tRNA fragments support survival of botulinum-intoxicated neurons by blocking ferroptosis
    Article Snippet: Botulinum neurotoxins (BoNTs) block cholinergic signaling at neuromuscular junctions, inducing transient muscle paralysis while avoiding neuronal death.. However, the mechanism(s) underlying these dual features are yet unknown.. Here, we report accumulation of 5’Lys transfer RNA fragments (tRFs) in both BoNT/A-intoxicated cultured human neuroblastoma cells and submandibular glands from BoNT/A-intoxicated rodents.

    Construct:

    Article Title: Co-exposure to polystyrene microplastics and glyphosate induces gut microbiota dysbiosis and cognitive impairment in honeybees.
    Article Snippet: • PS and GLY co-exposure showed more severe impacts on honeybee survival



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